Our Privacy Policy includes important information about our collection, use and disclosure of your personal information (including to provide you with targeted advertising based on your online activities). It explains that if you do not provide us with information we have requested from you, we may not be able to provide you with the goods and services you require. It also explains how you can access or seek correction of your personal information, how you can complain about a breach of the Australian Privacy Principles and how we will deal with a complaint of that nature.
The Ketogenic Diet is designed to force your body into ketosis, which is a normal metabolic state. Typically, the body burns carbohydrates from food to function, but when you adopt a diet of low calories (and low carbohydrates) your body switches into ketosis. When your body is in a state of ketosis the body is burning fat for energy, meaning you are tapping into the body’s fat storage that is often the hardest to shift.
Peptides are defined as a compound of two or more amino acids in which a carboxyl group of one is united with an amino group of another. With the elimination of a water molecule, a peptide bond is formed. To put it more simply, peptides are just small proteins. When the number of amino acids are less than 50, these are peptides. When higher than 50, these are proteins. The peptides are therefore small chains of amino acid which are present in all cells of the body. There are several kinds of peptides: oligopeptides, polymers, proteins, neuropeptides and peptide hormones. These are synthesized naturally by the body. Peptides will therefore be used primarily to make a more abundant amount of hormones in the body. The latter will then produce new hormones such as Testosterone or corticosteroids. It is these two hormones that are considered anabolic or indirect anti-inflammatory.
Solcoseryl: Derived from calves’ blood and is believed to speed up healing of damaged or injured tissues, solcoseryl is currently used in humans as eye gel for corneal ulcers, a jelly/ointment for gangrene and bedsores, burns and wound healing, and inflammation of gums, lips and mouth ulcers. No major adverse effects been reported. Solcoseryl is not specifically banned under WADA as a substance but can potentially be banned as a method depending on how the substance is administered and how much is used.
The peptide therapy protocols (Amino Acid Analogs) prescribed by TeleWellnessMD providers are also known as secretagogues (pronounced se-creta-gog), a substance that promotes secretion. These amino acid chains communicate with the body to produce or release growth hormone. Hence a secretagogue causes the body’s own natural processes to produce growth hormone. Secretagogues do not act as growth hormones but rather stimulate the pituitary gland to secrete your stored growth hormone. The subcutaneous injection route of growth hormone stimulation is a preferred route to help slow down age and environmental reductions in growth hormone levels.
Perhaps. As Dr Larkins weighs in: "It's a different kind of chemical and physiological manipulation that goes way beyond what I'd call conventional hormone therapy. It's the next stage of technology that's come along to try and help people enjoy quality of life." A stage with an effect apparently significant enough to risk a professional athletic career for.
Ironically, it only appears that the version of IGF-1 produced in your own muscle has any true anabolic effects. But nonetheless, many folks who’ve used IGF-1 claim to have experienced significant anabolic effects of injections. However, the only evidence for such anabolic effects have been shown in people who are already clinically deficient in IGF-1.
Tissues from ob/ob and lean mice were cut into 100-mg pieces and homogenized with 1 ml TRIzol (Life Technologies, Inc., Grand Island, NY) using a PolyTron homogenizer (Kinematica, Lausanne, Switzerland). The samples were incubated for 5 min at room temperature to permit the complete extraction of the RNA from the tissues. An aliquot of 0.2 ml chloroform was added to each milliliter of TRIzol. The method of RNA extraction was as described by the manufacturer (Life Technologies, Inc.). The final RNA pellet was air dried for 5–10 min and then resuspended in 20 μl ddH20. RNA was quantitated spectrophotometrically using the A260/280 ratio.
Between 2001 and 2006 six human clinical trials with the hexadecapeptide AOD9604 have been performed, 893 healthy, in all but one study, clinically obese adults participated in these studies and are the basis of this safety evaluation. The details of the individual studies are listed in supplementary data. The first 3 studies were dose-escalating studies investigating the acute effects of various dosages and two application routes (i.v. and oral) in healthy or obese male subjects. These single dose studies were followed by a 7-day multiple dose study (METAOD004) as well as two long-term clinical trials (METAOD005 and METAOD006) where the safety and tolerability of chronic oral treatment with AOD9604 was investigated.
Yes, Ipamorelin can help you lose weight. But, if you are not exercising, and aren’t eating well, it can only do so much. There is no magical supplement which will undo laziness and a horrible diet – keep this in mind. When using it for fat loss, make sure you are exercising. Doing so will naturally increase weight loss results, as you are going to burn more calories, along with the caloric deficit you are already on, for greater results. Further, your diet matters. If you are eating 5000 calories of junk per day, no supplement will help you lose weight – no matter how potent it claims to be!
A similar clinical study was conducted in obese subjects (METAOD002). In that double-blind placebo-controlled, 4 sequence, 4 period William’s Latin Square design study 23 subjects participated. The subjects were 19 to 50 years old and had a BMI ≥ 35 kg/m2 (range 36 to 67 kg/m2). Each subject received 4 single doses (25, 50 and 100 µg/kg AOD9604 or placebo; single IV infusion doses over 20 minutes), separated by a 7-day washout period.
Within all clinical trials the subjects underwent physical examination. The vital signs were observed, laboratory parameters were analyzed (hematology; biochemistry, urinalysis, lipid analysis), and ECG were measured before and after treatment (or in between and follow-up depending on the duration of the study). All subjects were interviewed at each visit with regard to any adverse events (AEs) they had experienced since the previous visit. The causality of AEs (namely their relationship to trial treatment) was assessed by the Principal Investigator. Special attention was made to on the evaluation of Serious Adverse Events (SAE).

CJC-1295 and Mod GRF 1-29 are administered in micrograms (mcg) rather than milligrams (mg) – the unit of administration of other steroids and performance-enhancing drugs. It has also been found that a 100mcg dose is enough to fully saturate the receptors in the anterior pituitary. This is called the saturation dose. After a dose of 100mcg has been administered, the subsequent dosages will achieve only half the effect.
Smeath says he turns patients away on a weekly basis. "[Particularly] if a patient comes and asks me for a specific peptide and it's not right for them. It's the same as if an anti-biotic was unsuitable for a patient, we don't just prescribe it." He says a lot of people prefer face-to-face contact with the prescribing doctor, but if geography restricts them, phone and Skype sessions also take place.

Other studies have had similar results with regards to the effect of collagen supplementation in helping to promote fullness. One study consisted of 24 healthy adults testing the satiety effects of various protein supplements. The subjects had two breakfast meals with specific types of protein in each: one meal had alpha-lactalbumin, gelatin, or gelatin + tryptophan (TRP) (Breakfast 1) and the other meal contained casein, soy, whey, or whey + glycomacropeptide (GMP) (Breakfast 2). The study found that Breakfast 1, which included gelatin (collagen), was 40% more satiating than Breakfast 2, which did not contain gelatin or collagen. Additionally, the participants who ate Breakfast 1 with gelatin ended up consuming less calories for lunch. Researchers concluded that gelatin increases satiety, which can lead to subsequent reduced energy intake, thereby promoting weight loss (2).
The male β3-KO mice and wild-type (WT) that were used in this study were offspring of animals provided by Dr. Bradford Howell (Beth Israel Hospital, Harvard Medical School, Boston, MA). The animals were bred and housed in the central animal house facility (Monash University). For chronic studies, animals were housed in the Departmental Animal Facility (Biochemistry, Monash University). For acute energy expenditure studies, animals were transported to the Department of Medicine (University of Melbourne), acclimatized, and killed following experimentation. The WT andβ 3-KO mice genotype were verified by breeding records and RT-PCR analysis performed in the laboratory of Professor Roger Summers.
Growth Hormone (GH) and IGF-1 are naturally occurring hormones in the human body responsible for many enviable aesthetic traits such as muscle mass, leanness and a firm/even skin tone. As people age, levels of growth hormone rapidly decline and this is one of the main reasons humans put on weight, lose muscle mass and develop sagging/uneven skin. It's no surprise then that synthetic Human Growth Hormone is a sought after product for anti-aging by persons looking to remain youthful, bodybuilders looking to put on muscle mass and people in general who are looking to "tone up" or lose stubborn belly fat.
Studies have shown that individuals fighting infection have a lower amount of circulating T α 1 and suppressed helper T cell numbers compared to healthy individuals. This is problematic, as optimal immune function is vital to recovery from infection. Supplementation with T α 1 has the potential for great therapeutic benefit for patients suffering from infection or autoimmune disease.
OxySelect Pink is an advanced thermogenic and is the only diet pill specifically formulated to enhance the curves of a woman’s body. While other products contain stimulants and huge quantities of dubious fillers and preservatives, OxySelect Pink uses clinically-tested, all-natural extracts designed to help you lose weight. OxySelect Pink is one of the best choices for women looking to get leaner.
Various experiments have been conducted to test the effectiveness of CJC 1295-DAC in vivo and the Journal of Clinical Endocrinology & Metabolism has reported dose-dependent increases in mean plasma GH concentrations by 2-10 fold for more than 6 days and increased IGF-1 concentrations 1.5-3 fold for 9-11 days after a single injection. Mean half-life was shown to be 5.8-8.1 days, also after multiple doses mean IGF-1 levels remained above baseline for up to 28 days. No serious adverse reactions were reported in any group.
The expression of β3-AR RNA was assessed by RT by using radiolabeled primers and Southern blot analysis. Labeled bands were identified and semiquantitated by phosphorimaging. RT-PCR analysis demonstrated that ob/ob mice express lower amounts ofβ 3-AR RNA in their white (Fig. 3A) and brown (Fig. 3B) adipose tissues, compared with lean C57BL/6J mice, in agreement with others (14). Figure 3A shows the effect of saline, AOD9604, and hGH on β3-AR RNA expression in epididymal adipose tissue from both lean and obese mice, respectively. The level of β3-AR expression increased significantly in response to AOD9604 and hGH only in the obese mice, correlating with the significant decrease in epididymal adipose tissue weights seen in these mice. The same correlation was observed in brown adipose tissue, where increased expression of β3-AR was accompanied by a decrease in brown adipose tissue mass in both lean and obese mice (Fig. 3B).
For example, there are Growth Hormone Releasing Peptides (GHRP’s with names such as ipamorelin and hexarein) which allow for a slow and steady growth hormone release that produces a pulse which mimics natural growth hormone release times and Growth Hormone Releasing Hormones (GHRH’s such as Mod-GRF) for an even stronger natural release of growth hormone and greater presence of growth hormone precursors known as GH “frags” or fragments.
Causing one to be " hungry as a wolf " and having a strong gastric motility, this 1er injectable peptide is interesting for fitness practitioners who may require support to finish their carefully prepared meals. This sudden increase in appetite that occurs around 20 minutes after the injection is related to the fact that GHRP-6 stimulates the release of a digestive hormone, Ghrelin, which is designed to cause hunger pangs. This makes it a valuable ally in weight gain treatments , which is important.
After about a week of taking the collagen, I noticed my nails were thicker and stronger and were growing faster. Usually, my nails break and tear off after they grow, but after drinking my collagen, they were growing nicely and faster than normal. In fact, when I got a gel manicure, which is supposed to last up to two weeks, my nails grew out after about eight or nine days, leaving the bottoms of my nail beds exposed. Although it was annoying having to get my nails redone, it wasn’t a bad problem to have!

To really test the health benefits of drinking collagen peptides, I vowed to take it every day for two weeks. I opted for Vital Proteins Unflavored Grass-Fed Collagen Peptides in the blue tub. I got the 20-ounce tub and a box of the stick packs, which were perfect for when I went out of town. I stuck with one scoop from the tub for 35 calories, 9 grams of protein, and 10 grams of collagen (this was the same serving size in the stick packs).
One of the biggest concerns many of us have as we get older is: weight management. Maintaining a healthy weight is a lifelong struggle for many and can get harder as we get older. In fact, statistics show that 70% of American adults are overweight, and half of those adults are obese. We need to find ways to lose weight in a healthy manner, and more importantly keep off the weight, long-term. Ongoing research about collagen, a natural and unique type of protein, shows that collagen supplementation just might be the key in your journey to stay at a healthy weight and better your health.
Diets on point! 6 600 calorie meals totaling 200-300g protein, 50-150g carbs and almost NO fat. I do get a little fat in my diet from flax, trail mix and what not but all my food is natural, whole and as close to organic as I can get.. TONS of raw veggies. NO cardio atm, Ive got some bad injuries and issues developed from my last cycle so I take it pretty easy LOL. Maybe an hour on the bicycle 1-2x/wk.. Tough job tho so at work 8hrs a day working on diesel pile hammers keeps my heart rate pretty high all day
Peptide therapy, or the use of specific peptides in treatment, has gained great popularity in recent years. This is due largely to the fact that these peptides are highly specific (i.e., only do what you want them to do) while also being well-tolerated and safe. As of January 2015, there were over 60 US FDA-approved peptide medications, 140 peptide drugs being evaluated in clinical trials, and 500 in pre-clinical development.
In order to demonstrate safety, several human studies were performed with AOD9604 (supplementary data): 1). METAOD001: A Phase I (double-blind, placebo-controlled, dose escalation) safety study with doses (ranging from 25 to 400 µg/kg AOD9604) administered intravenously to 15 healthy adult male volunteers presenting with a BMI between 24 and 30 kg/m2. A single dose of recombinant hGH (0.12 international units/kg) was administered intravenously as positive control. 2). METAOD002: A Phase IIa (double-blind, placebo-controlled 4 × 4 Latin Square design) safety study with single doses (25, 50 and 100 µg/kg AOD9604) administered intravenously to 23 healthy clinically obese males presenting with a BMI ≥ 35 kg/m2. 3). METAOD003: A Phase IIa (double-blind, placebo-controlled 4 × 4 Latin Square design) safety study with single doses (9, 27 and 54 mg AOD9604) administered orally (capsules) to 17 healthy, clinically obese males presenting with a BMI ≥ 35 kg/m2. 4). METAOD004: A Phase IIa (double-blind, placebo-controlled, dose escalation) safety study with multiple daily doses (9, 27 or 54 mg AOD9604) administered orally (capsules) for seven days in 36 healthy clinically obese males presenting with a BMI ≥ 30 kg/m2. 5). METAOD005: A Phase IIb (randomized, double-blind, placebo-controlled) study to assess the efficacy (reduction in body weight), safety and tolerability of 12 weeks treatment with daily doses (1, 5, 10, 20 or 30 mg AOD9604) administered orally (capsules) in 300 healthy, clinically obese males, and females of non-child bearing potential, with a BMI ≥ 35 kg/m2. 6). METAOD006: A Phase IIb, randomized, double-blind, placebo-controlled study to assess the efficacy (reduction in body weight), safety and tolerability of 24 weeks treatment with different doses of AOD9604 tablets (0.25 mg, 0.5 mg, 1 mg, or placebo) in 502 obese adults.
The evidence so far suggests that there may be some effect on cartilage and bone densities, and it is correlated with weight loss. I understand that there is no evidence to suggest that it stimulates the release of IGF-1, which is the mechanism via which growth hormone gets most of its purported performance enhancing effects (i.e. muscle bulk). Whilst this is a decent list of things to test if one is considering AOD9604 as simply a variant of growth hormone, it is by no means exhaustive of all the mechanisms via which a drug can enhance athletic performance. The evidence thus far suggests that AOD9604 could be performance enhancing, but there is nothing I would hang my hat on. That is why I maintain that Dank is more sorcerer than scientist.
2. If HGH can be obtained legally from a physician for anti-aging it's extremely expensive, with 10iu (units) costing as much as $200USD. As a comparison, the 10iu equivalent of GH releasing peptides (approximately half of one vial) sells online for approximately $20USD, a full 90% cheaper than actual HGH for the same, if not better, positive effects on the body.
Our peptide therapies are also known as secretagogues – a substance that promotes secretion.  These amino acid chains communicate with the body to produce or release growth hormone.  The increased volume of human growth hormone produced by the pituitary gland causes an increase in the production of Insulin-Like Grow Factor-1 (IGF-1) by the liver and results in several health benefits such as:
PCR amplification was carried out on cDNA equivalent to 100 ng of starting mRNA using the following murine oligonucleotide primers (expected and observed PCR product size): β3-AR forward, 5′-TCTAGTTCCCAGCGGAGTTTTCATCG-3′; (234 bp) reverse, 5′-CGCGCACCTTCATAGCCATCAAACC-3′; β-actin forward, 5′-ATCCTGCGTCTGGACCTGGCTG-3′; (559 bp) reverse, 5′-CCTGCTTGCTGATCCACATCTGCTG-3′.

AOD9604, a synthetic fragment of hGH consisting of the amino acid residues 177–191 with the addition of a tyrosine residue to the N-terminus was prepared with solid-phase synthesis procedure and purified with reverse-phase HPLC methodology in our laboratories (13). The structure of the peptide analog was verified with mass spectrometry and amino acid analysis. The hGH was a gift of Professor Michael Waters (University of Queensland, Brisbane, Australia). BRL37344 (β3-AR agonist) was a gift from Dr. Jon Arch (SmithKline Beecham, Harlow, UK).


Your level of physical activity also affects IGF-1, and heavy weight training for your legs is a particularly potent way to increase it. Some studies suggest that the effects of the popular anti-aging supplement DHEA actually arise due to this same type of increase in IGF-1 in the body that occurs with with weight training (so you choose: heavy barbell squats or a bottle of DHEA from the drugstore).


Monash University discovered that the fat-reducing effects of GH appear to be controlled by a small region near one end of the GH molecule. This region, which consists of amino acids 176-191, is less than 10% of the total size of the GH molecule and appears to have no effect on growth or insulin resistance. It works by mimicking the way natural Growth Hormone regulates fat metabolism but without the adverse effects on blood sugar or growth that is seen with unmodified Growth Hormone. Like Growth Hormone, AOD 9604 stimulates lipolysis (the breakdown or destruction of fat) and inhibits lipogenesis (the transformation of nonfat food materials into body fat) both in laboratory testing and in animals and humans. Recent findings have shown, in addition to its fat loss properties, AOD 9604 processes many other regenerative properties associated with growth hormone. Currently trials are underway to show the application of AOD 9604 in osteoarthritis, hypercholesterolemia, bone and cartilage repair. AOD 9604 has an excellent safety profile, recently obtaining Human GRAS status in the USA.
CJC-1295 is basically a peptide hormone that acts similar to growth hormone releasing hormones (GHRH). Invented by a Canadian biotechnology company called ConjuChem, it is beneficial to athletes because it can bioconjugate with circulating albumin and increase the time it can be used for medical purposes. It achieves this by preventing degradation of its amino acids. With a single dose, it can remain in the body for quite a few days and can cause the growth hormone to be released many times per day. This reduces the frequency of injections needed.

It has to be noted that three of the SAEs were skin cancer forms. Since the study was performed in Australia, a country with the highest incidence rate of skin cancer (http://globocan.iarc.fr/), this cumulative incidence is not improbable. Furthermore the study was performed on clinically obese subjects with a BMI ≥ 35 kg/m2 (BMI ≥ 35 kg/m2; Median BMI: 40 kg/m2, range: 35 to 67 kg/m2). It is known that the incidence of several types of cancers is associated with increased BMI [26].
Advanced Obesity Drug 9604 (AOD9604), targets fat metabolism directly by enhancing the breakdown of stored fats and inhibiting the synthesis of new fats. It is a peptide drug derived from the fat-reducing activity of human growth hormone (hGH). hGH has been shown to cause fat reduction, but is not suitable as an anti-obesity drug as it also causes an increase in muscle, organ and bone mass as well as causing diabetes to develop. The effectiveness of hGH declines in older people, correlating with the tendency to put on weight as one gets older. In support of this, the clinical trials so far have shown a greater effect of AOD9604 in the older population.
It is well known that hGH is associated with increased IGF-1 levels. IGF-1 may have a variety of undesirable effects, including an increase in cancer risk [22, 23]. In the studies discussed herein, IGF-1 levels were monitored in all long-term studies but did not reveal clinically significant differences between dose groups or placebo. Therefore the 5 SAEs that occurred in the 12 week treatment study (three in the AOD9604 20 mg group (basal cell carcinoma, moderate lipoma and squamous cell carcinoma), one in the 10 mg group (malignant melanoma) and one in the 5 mg group (breast cancer)) could not be attributed to increased IGF-1 levels. The Principal Investigator considered none of the reported SAEs to be “possibly”, “probably” or “definitely related” to the study medication. The rationale behind this judgment was that none of the cancer forms occurred in the highest dosage group (30mg AOD9604/day), therefore a dose effect can be excluded. Further examination of the SAE cases indicated that these subjects had neglected their personal medical care for a longer period of time, so that the higher incidence of cancer may well have occurred due to the natural incidence rate of cancer events in the population.
Our hormone levels decline as we age, and therefore the effects of these hormones decline proportionally. Even if you exercise and eat well, you will still experience this decline in hormone production and all of the associated adverse health effects that this brings. To fight ageing, and increase vitality, we can restore our hormones to their youthful levels.
The biggest negative, and this is a big one, is that AOD9604 has undergone very rigorous scientific testing, and has been found to have no effect in humans (3). When AOD9604 was first developed, it showed significant promise as a weight loss treatment. A special strain of obese mice supplemented with the peptide showed a reduction in weight, increased fat oxidation, and raised plasma glycerol, which are indicators of lipolysis, or fat burning (5). Subsequent studies in obese mice and rats attempted to show that the peptide works to burn fat in the same way as human growth hormone, but found that this was not the case, meaning that the fact this peptide resembled hGH was meaningless. Scientists were unable to determine how this peptide was working in mice (6).
Endurobol (GW501516): Classified under a group of drugs called peroxisome proliferator-activated receptor (PPAR) agonists, Endurobol’s potential abuse in athletes is based on animal studies that showed it could improve endurance, increase fat metabolism, improve glucose uptake in skeletal muscle tissue, and increase in muscle gene expression. At the moment, there is insufficient evidence for these sport performance outcomes in humans. Human side-effects are currently also unknown. Endurobol is prohibited both in and out of competition under WADA’s Prohibited List.
It is also important to note that whether you are a long-time user or a first-time user of Ipamorelin, your body is going to react differently to that of the next user. Like the benefits you will experience, the side effects you are going to experience will occur differently, and at different dosage levels. So, it truly is a trial and error period you are going to go through with a test run of Ipamorelin for new users. You have to find what works for you, how your body will react, and what potential side effects are lingering ahead, in order for you to achieve the greatest results, and eventually find the proper dosage and cycle level, which is going to work the best for your body and system.
For example, studies have shown that people deficient in IGF-1 have an increased chance of dying from a heart attack. This is because IGF-1 prevents the death of heart cells and offers protection to heart cells when the cells are stressed, such as during a heart attack or long amount of time without oxygen. IGF-1 has a similar protective effect on brain cells.
Fenphedra could have easily been our #1 rated diet pill for women if not for its safety concerns. There have been rumors Fenphedra contains phentermine and prescription-strength pain relievers. Any rumors of such claims are completely false, but Fenphedra does contain phenylethylamine. Often referred to as the “Love Drug,” phenylethylamine is extracted from cocoa and responsible for the “chocolate high” sensation. Fenphedra also contains Green Coffee Bean, Chromax and Dicaffeine Malate, which dramatically burn fat, suppress appetite, and increase energy. Finding bottles of Fenphedra in the U.S. can be difficult because rumors are flourishing that it may be taken off the market like banned diet pills for women. Fenphedra retails for $130 per bottle and comes with a 100% money-back guarantee. The cheapest place to buy online occasionally has bottles available for as low as $69.95. Read More
Very tough to say. I am not a doctor and this is not to be taken, interpreted or construed as medical advice. Please talk with a licensed medical professional about this. These are just my own personal thoughts and not a prescription or a diagnosis or any form of health care whatsoever. I could possibly help but would need to see your health history, blood, biomarkers, etc. I'd be happy to help you via a personal one-on-one consult. Just go to https://bengreenfieldfitness.com/coaching. and then choose a 20 or 60 minute consult, whichever you'd prefer. I can schedule ASAP after you get that.
AOD aka Advanced Obesity Drug., Fat Loss Peptide AOD 9604 consists of the get along 15 amino acids of the human wealth hormone molecule. The considerable thing close but no cigar AOD 9604 is it has no doom on accomplishment or insulin resistance. With an fine safety sketch of minimal side chattels personal, AOD 9604 has been proven more effective than consequently occuring Growth Hormone at fresh the collapse of fat. Studies have proven its efficiency to reduce biggest slice of the cake full, by way of explanation in the abdominal area.
Ultimately, it needs to be trialled in athletes. But that would never happen. As a substance with no therapeutic use, there is no need to ever test it to determine its effect on athletic performance. You don't need the same standard of evidence for drugs like EPO (synthetic replicas of hormones), where the primary biological effect studied in clinical trials of non-athletes is clearly and blatantly performance enhancing. With something like AOD9604, the waters are much murkier with respect to athletic performance.

cDNAs were synthesized by reverse transcription (RT) of 1.0 μg of each total RNA using oligo (dT)15 as a primer. The RNA in a volume of 7.7 μl H2O was heated to 70 C for 5 min then placed on ice for 2 min before the addition of a reaction mix containing 1× RT buffer (Promega Corp., Madison, WI), 1 mM deoxynucleotide triphosphates (dNTPs), 5 mM MgCl2, 18 U Rnasin (Promega Corp.), 20 U avian myeloblastosis virus RT (Promega Corp.), and 50 μg ml−1 oligo(dT)15 in a volume of 12.5 μl. Following a brief centrifugation, the reactions were incubated at 42 C for 45 min and then 95 C for 5 min. The completed RT reactions were stored at −20 C and used for PCR without further treatment.
When combined with the other IGF-1 and growth hormone boosting strategies you’ve just discovered – such as eating adequate calories, heavy weight training, 7-9 hours of sleep per 24 hour cycle, adequate mineral intake and moderation of alcohol intake – these additional strategies will ensure you get all the anabolic effects of IGF-1 and growth hormone without having to resort to needles, syringes, prescriptions, online pharmacies and potentially dangerous self-experimentation.
Comparing everything else is hard due to branding... don't want to start going into branding for obvious reasons. GH is incredible for fatloss but some brands add so much water it is hard to see the results to begin with. But due to the high serum and igf-1 from gh excellent results are to be had. Many factors come into play though which I am sure your well aware of. I think the ghrp's (2 and 6 for example) are excellent for fatloss if you keep up with the 4 daily injections in your research. Otherwise they are nowhere as effective due to the short peak times... so one must be organized and disciplined to get the full benefits.
Other peptides amplify the body’s response to its own testosterone site-specifically within muscle and bone only. Steroids, on the other hand, produce their highly desired anabolic effects by acting on these receptors, but also come coupled with the less-sought-after side effects of acne, oily skin, hair loss, aggression, breast enlargement and testicular shrinkage. It is for this reason, these amino acid chains are serving as an increasingly popular alternative to steroids amongst aesthetic gym goers, providing many of the anabolic effects without the unwanted side effect profile.
×