HUMAN GH (hGH) has profound lipolytic/antilipogenic actions in vivo, which result in decreased fat mass, increased lean mass, and weight loss (1–3). Studies in vitro and in vivo have indicated that this response is mediated in part by an increase inβ -adrenoceptor coupling efficiency (4), a reduction in Gi expression (5), increased activity of hormone sensitive lipase (6), and an inhibitory effect on the action of insulin (7). We have synthesized a fragment of hGH (AOD9604) that contains a lipolytic domain that may be responsible for the lipolytic action of hGH. The parent molecule, AOD9401, induces lipolysis and antilipogenesis and fat oxidation in adipose tissue in vitro (8, 9). In vivo, AOD9401 induces weight loss without affecting food intake as well as increasing lipolytic sensitivity and increasing fat oxidation with no adverse effects on insulin sensitivity (8, 10).


When you are just getting started with Ipamorelin, it is advised to use only one supplement daily at the same time each day. It is also advised to begin on the lower end, typically an eight-week cycle, and at a maximum twelve-week cycle. Doing this not only guarantees the desired results when using Ipamorelin, it is also going to ensure you get the most out of the supplement. When using this dosage cycle you will:
AOD9604 and hGH appear to act in a similar manner to induce their effects on body weight regulation and adipose tissue mass in vivo. However, in vitro studies have demonstrated a number of differences suggesting that the two compounds operate via unique signaling pathways to control the regulation of theβ 3-AR. These studies suggested that AOD9604 had no interaction with the β3-AR or hGH receptors (11).

Amongst its metabolic effects, hGH can induce inhibition of lipoprotein lipase activity in adipose tissue, stimulating lipolysis in adipocytes, which results in the reduction of fat cell mass [4-7]. Moreover, a correlation has been found between adiposity and the reduced circulating levels of hGH [8]. When applied systemically, hGH reduces body fat mass and influences fat distribution [9]. Therefore, treatment with hGH should theoretically have a positive impact on obesity. However, long term treatment with hGH is associated with various health risks, including glucose intolerance and insulin resistance, diabetes, acromegaly, cancer, edema, and hypertension [10-13].
The Ketogenic Diet is designed to force your body into ketosis, which is a normal metabolic state. Typically, the body burns carbohydrates from food to function, but when you adopt a diet of low calories (and low carbohydrates) your body switches into ketosis. When your body is in a state of ketosis the body is burning fat for energy, meaning you are tapping into the body’s fat storage that is often the hardest to shift.
When taking Ipamorelin, you want it to be pushed through your system naturally, and at the same levels. If you are constantly altering the times you take it, or increase/decrease dosages during your cycle, this is not going to be attainable. To maximize the benefits and gains you are going to experience, dosage levels should be consistent, as should the timing of the dosage you are taking each day.
Melanotan II is an analogue of alpha melanocyte stimulating hormone, the hormone responsible for pigmentation in skin and hair. This peptide has been shown not only to increase skin pigmentation, resulting in a substantially tanner skin tone, but also to stimulate fat loss and increase libido. Its aphrodisiac effects were so substantial that it was the basis for the development of another peptide designed exclusively to address erectile and sexual dysfunction—Bremelanotide PT 141.
PCR amplification was carried out on cDNA equivalent to 100 ng of starting mRNA using the following murine oligonucleotide primers (expected and observed PCR product size): β3-AR forward, 5′-TCTAGTTCCCAGCGGAGTTTTCATCG-3′; (234 bp) reverse, 5′-CGCGCACCTTCATAGCCATCAAACC-3′; β-actin forward, 5′-ATCCTGCGTCTGGACCTGGCTG-3′; (559 bp) reverse, 5′-CCTGCTTGCTGATCCACATCTGCTG-3′.
The effect of a single daily ip dose of saline, AOD9604, or hGH on body weight changes in lean male C57BL/6J (A) or obese (ob/ob) mice (B) for 14 d. Caloric intake was recorded every second day and presented as an average for each day in lean (C) and obese (D) mice. Results are expressed as the mean ± SE of six animals in each group. *, P< 0.01; #, P < 0.05, compared with saline.
Metabolic has just completed Phase IIA studies in a group of 22 clinically obese, but otherwise healthy, male patients ranging in age from 22?50. The results showed that the drug was well tolerated and increased fat metabolism within two hours of administration by 25 per cent in the older group of patients. Although not the focus of the study, weight loss was also demonstrated, particularly in the older group.

The peptide therapy protocols (Amino Acid Analogs) prescribed by TeleWellnessMD providers are also known as secretagogues (pronounced se-creta-gog), a substance that promotes secretion. These amino acid chains communicate with the body to produce or release growth hormone. Hence a secretagogue causes the body’s own natural processes to produce growth hormone. Secretagogues do not act as growth hormones but rather stimulate the pituitary gland to secrete your stored growth hormone. The subcutaneous injection route of growth hormone stimulation is a preferred route to help slow down age and environmental reductions in growth hormone levels.


The effect of AOD9604 treatment on IGF-1 levels was of particular interest, since hGH administration is associated with increases in IGF-1 levels. Prolonged increases in IGF-1 may have a variety of undesirable effects, including an increase in cancer risk [22, 23]. In this study the levels of circulating IGF-1 were measured at the baseline visit and at the end of the 12-week treatment period. There was no significant change in the levels of IGF-1 in any of the treatment groups (Table 1).
The original GRF (1-29) has a half-life of about 30 minutes. Half-life means the time within which half of the hormone administered will be destroyed within the body. This short half-life is due to the fact that the compound is highly unstable and breaks down soon. To increase its stability and to make it last longer, it was modified by adding 4 amino acids in its structure. This gave it the name Modified GRF (1-29) or Mod GRF 1-29. It was originally invented by DatBtrue. The portion of the molecule that actually stimulates the growth hormone secretion is found in the chain of 29 amino acids, so it is named GRF (1-29). This chemical also produces slow-wave sleep.

Finally, the hexadecapeptide AOD9604 did not induce allergenic reactions when consumed over 24 weeks. Blood of patients was analyzed for the presence of anti-AOD9604 antibody formation at various times and at the end of the studies (latest time point after 24 weeks). In none of the performed studies, at no time, were anti-AOD9604 antibodies detected in serum collected from any subjects in any treatment group.
CJC1295: A growth hormone-releasing peptide, CJC1295 was first made by a Canadian biotechnology company to reduce fat deposits in obese AIDS patients. Research has shown that almost 100% of people injected with CJC1295 experienced side effects such as high blood pressure, diarrhoea, and headaches. There is no published evidence that CJC1295 produces any benefit to athletes. There is little or no peer-reviewed evidence that CJC1295 gives any advantage in sports, and as a growth hormone-releasing substance, is banned by WADA.
The effects of hGH and AOD9604 on fat metabolism may be mediated by an alteration in the expression of a lipolytic/antilipogenic gene. Theβ 3-AR is a major lipolytic receptor identified in rodent fat cells (18) that mediates its effects through G protein coupling to adenylate cyclase, generation of cAMP, and stimulation of PKA (19). This enzyme then phosphorylates proteins in the lipolytic cascade, including hormone-sensitive lipase (20). In BAT, the β3-AR stimulates uncoupling of the electron transport chain, enhancing the ability of mitochondria to generate heat in preference to ATP through the dissipation of the electron gradient (21). Mice that lack this receptor have lower rates of resting energy expenditure (0.0041 vs. 0.0047 kcal/min, P < 0.02) and lower rates of fat oxidation (0.00019 vs. 0.00030 g/min, P < 0.02) than control mice (data not shown).
Despite the controversies, some scientists continued with additional studies and again proved IGF-1 to actually prolong life…at least in worms.  Then, in 2001, scientists discovered that the use of IGF-1 resulted in a proliferation of cancer cells, especially throughout the breast and colon, and a 2012 study found that both too much or too little IGF-1 could contribute to dying from cancer; implying that IGF-1 actually helped patients with terminal cancer live longer.

Prior to commencement of active treatment, 48.4% of subjects experienced at least one AE. The body system organ classes with the highest incidences of events (> 10%) were the nervous system (17.5%; mainly headache, 14.5%), infections and infestations (15.9%, mainly nasopharyngitis and upper respiratory tract infection, 4.0%), gastrointestinal system (12.4%, mainly diarrhea 3.2%) and musco-skeletal and connective tissue disorders (12.0%, mainly back pain, 4.0%), 32.9% of subjects experienced mild AEs, 38.6% experienced moderate AEs and 36 (7.2%) patients experienced severe AEs. The intensity of AEs was similar across all treatment groups. None of the AEs were deemed to be definitely related to the study treatment.

AOD9604 and hGH appear to act in a similar manner to induce their effects on body weight regulation and adipose tissue mass in vivo. However, in vitro studies have demonstrated a number of differences suggesting that the two compounds operate via unique signaling pathways to control the regulation of theβ 3-AR. These studies suggested that AOD9604 had no interaction with the β3-AR or hGH receptors (11).
A total number of 207 AEs were reported by 36/36 subjects. All but one was of mild to moderate intensity (placebo-treated subject, soft tissue injury to left shoulder, unrelated to study treatment). No SAE occurred during the 7-day treatment and the 7-day follow-up time. The rate as well as the AE profile was comparable in the 9 mg, 27 mg AOD9604 and the placebo group. There was no observable trend between treatment groups with respect to the incidence of certain AEs, however subjects who received 54 mg AOD9604 experienced a greater number of headaches, diarrhea and flatulence.

Both human GH (hGH) and a lipolytic fragment (AOD9604) synthesized from its C-terminus are capable of inducing weight loss and increasing lipolytic sensitivity following long-term treatment in mice. One mechanism by which this may occur is through an interaction with the beta-adrenergic pathway, particularly with the beta(3)-adrenergic receptors (beta(3)-AR). Here we describe how hGH and AOD9604 can reduce body weight and body fat in obese mice following 14 d of chronic ip administration. These results correlate with increases in the level of expression of beta(3)-AR RNA, the major lipolytic receptor found in fat cells. Importantly, both hGH and AOD9604 are capable of increasing the repressed levels of beta(3)-AR RNA in obese mice to levels comparable with those in lean mice. The importance of beta(3)-AR was verified when long-term treatment with hGH and AOD9604 in beta(3)-AR knock-out mice failed to produce the change in body weight and increase in lipolysis that was observed in wild-type control mice. However, in an acute experiment, AOD9604 was capable of increasing energy expenditure and fat oxidation in the beta(3)-AR knock-out mice. In conclusion, this study demonstrates that the lipolytic actions of both hGH and AOD9604 are not mediated directly through the beta(3)-AR although both compounds increase beta(3)-AR expression, which may subsequently contribute to enhanced lipolytic sensitivity.
As an athlete, you can also increase your dosage cycle for a period of 12 to 16 weeks at a time, to maximize your gains. Do so gradually if you opt to go this route. Make sure you increase your daily dosage (1 to 2 doses per day, etc.) gradually. Start off with lower dosage levels as well, and see how it interacts with your body. You don’t want to experience withdrawal, nor do you want to experience negative side effects when using Ipamorelin for longer dosage cycles. So, make sure you monitor your progress, see how you feel as you go, and make notes if/when you do experience negative side effects, so you can balance down to the proper dosage levels.
HUMAN GH (hGH) has profound lipolytic/antilipogenic actions in vivo, which result in decreased fat mass, increased lean mass, and weight loss (1–3). Studies in vitro and in vivo have indicated that this response is mediated in part by an increase inβ -adrenoceptor coupling efficiency (4), a reduction in Gi expression (5), increased activity of hormone sensitive lipase (6), and an inhibitory effect on the action of insulin (7). We have synthesized a fragment of hGH (AOD9604) that contains a lipolytic domain that may be responsible for the lipolytic action of hGH. The parent molecule, AOD9401, induces lipolysis and antilipogenesis and fat oxidation in adipose tissue in vitro (8, 9). In vivo, AOD9401 induces weight loss without affecting food intake as well as increasing lipolytic sensitivity and increasing fat oxidation with no adverse effects on insulin sensitivity (8, 10).

The final study involved an assessment of the acute effect of AOD9604 and a β3-AR agonist (BRL37344) on energy expenditure, fat oxidation, and glucose oxidation in WT andβ 3-KO mice. When AOD9604 or BRL37344 were administered to WT mice, an acute increase in fat oxidation and energy expenditure occurred, with an associated reduction in glucose oxidation (Fig. 6A). The effect plateaued 18 min following injection and remained stable for the duration of the experiment. The response to the two compounds was very similar, despite the fact we have previously shown that AOD9604 does not directly interact with the β3-AR as demonstrated by ligand binding studies (11). This clear separation of pathways was further confirmed in Fig. 6B in which AOD9604 clearly increases fat oxidation and energy expenditure inβ 3-KO mice, whereas BRL37344 does not. The KO mice neither decrease their glucose oxidation in response to AOD9604 nor show a prolonged increase in fat oxidation and energy expenditure in response to AOD9604.
If muscle gain is not what you’re looking to gain, then the best place to start is by getting your hands on some CJC-DAC and administer at 2-5mg per week. CJC-1295 with or without DAC is suitable. An equally effective alternative is GW501516. Peptide Clinics Australia recommend Oxytocin for weight loss highly and they’ve had amazing results from patients that have been prescribed this.
These results also demonstrate that, unlike hGH, AOD9604 has no negative effect on carbohydrate metabolism. This was previously demonstrated in mice. Chronic administration of hGH to ob/ob mice depressed glucose oxidation and increase plasma glucose levels. AOD9604 had no such effects, no changes in circulating plasma glucose in either lean nor obese ob/ob mice have been observed [20]. AOD9604 did not cause hyperglycemia or affect insulin sensitivity in rats and mice [18, 20].
I stopped the colostrum and my ” symptoms ” subsided, seems I have a moderately enlarged prostate which doesn’t run in my family on either side, my question is could the colostrum possibly cause the prostate to enlarge due to the igf-1 at a certain age,? due to a possible decline in testosterone, or could the benefits of colostrum outweigh the prostate issue?

For Growth Hormone (GH) to perform its anabolic (muscle building) affects it requires the presence of the body's most anabolic hormone: insulin. This is in contrast to GH related fat loss which requires insulin to be absent. However, since GHRP and fast-acting GHRH (Growth Hormone Releasing Hormone) products (i.e. Modified GRF 1-29) still need time to stimulate the body to release GH from the pituitary gland, the insulin spike must come after the injection and not before, otherwise the GH release will be blunted.
It is well established that hGH is a lipolytic hormone (15), but the exact mechanisms used are still unclear. In this paper we present data that suggest that hGH and its lipolytic fragment (AOD9604) induce their chronic in vivo actions on lipolysis in part by modulating the expression of theβ 3-AR. Human GH has been shown to affect the in vivo expression and function of β-ARs in vivo in sheep (16). Data presented in this paper indicate that chronic administration of hGH influences expression of the β3-AR in adipose tissue in the ob/ob mouse. In brown adipose tissue (BAT), these compounds also increase expression of β3-AR expression in the lean C57BL/6J mouse. The increase in expression induced by chronic hGH or AOD9604 treatment correlated with the decrease in adipose tissue mass. We therefore hypothesize that treatment with either hGH or AOD9604 enhances β3-AR expression, which has been observed in murine 3T3-F442A and human SK-N-MC cells in vitro (11).
A comparison of β3-AR RT-PCR expression levels in white (A) and brown (B) adipose tissues from lean and obese mice treated for 14 d with saline, AOD9604, or hGH. Results are displayed as a percentage, compared with lean controls, and expressed as the mean ± SE of three determinations in each group. *, P < 0.05; #, P< 0.05 obese control vs. lean control.
When using any GHRH, it should always be remembered that positive results cannot be achieved overnight. These compounds act steadily over time, and the best results can be achieved slowly, and with a nutritious diet and a proper exercise regime. Also, these peptides are not sex-specific, so they do not have any androgenic effects. They can be used by women in the same dosages that men do.
People who are serious about losing weight or improving physical performance may choose to use some of the controversial peptides. They are generally sold for research purposes, but many injectable forms of peptides have been used by athletes to increase the production of their body’s HGH to achieve increased lean body mass, decreased body fat, and improved recovery time after workouts. Peptides used for these purposes include:
In June 2007, the NDPSC decided to extend the exemption from the limit on paracetamol combinations being allowed as general sale products to include phenylephrine (as long as it also qualified as exempt from scheduling through the phenylephrine entries). At that time, the NDPSC considered that the safety profile of these substances was such that allowing a fixed combination to be unscheduled was reasonable.
What may be unknown to some people is that hormones can come in three classes. We are all familiar with steroid hormones, which are fat soluble hormones that include testosterone, oestrogen, and their derivatives. A lesser known group of hormones are those made from peptides. The best known peptide hormone is human growth hormone (hGH) and insuline-like growth factor 1 (or IGF1), both of which are anabolic hormones that are responsible for cell growth and proliferation. Finally, some single amino acid derivatives can also be classed as hormones. Amino acids such as phenylalanine, tyrosine, and tryptophan can also be biologically modified into hormones.
Mostly, these peptides are sold as lyophilized powder in 2mg containers. Bacteriostatic water should be mixed with the powder in order to reconstitute it. To make the dosage of 100mcg per injection, 2ml bacteriostatic water should be mixed into 2mg of lyophilized powder. This reconstituted mixture should be then injected inside the muscles or under the skin. The mixture should be kept under refrigeration at all times otherwise it will degenerate and will not be effective anymore.

In addition, scientists have observed that those who supplemented with IGF-1 experienced a preponderance of new brain cell growth and new muscle mass and new studies confirmed this to be true, even among individuals with both brain damage and muscle-wasting sarcopenia. Furthermore, research studies have found IGF-1 to increase feelings of youthfulness, improve well-being, and even to alleviate depression.

Peptide Clinics is an Australian owned and operated company, based in Sydney, that specialises in providing premium peptides online, coupled with expert medical guidance in their safe and effective use. Under the supervision of our experienced hormone doctors, Peptide Clinics Australia services thousands of clients throughout Australia with great success.
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